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病毒感染RAW
慢病毒载体感染肿瘤细胞建系后做裸鼠成瘤,但基因片段很长怎么办?
慢病毒载体,腺病毒载体,腺相关病毒载体(AAV)在应用上有什么区别?各自有什么有缺点?
质粒转染和病毒载体介导转染的区别?各自优缺点?
腺病毒怎么纯化?
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siRNA有效果,包成shRNA的慢病毒就没有效果了,这是为什么?
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2020-09-08
siRNA合成种的OPC纯化,PAGE纯化,HPLC纯化这三种纯化有什么区别?
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1回答 3612浏览
2020-08-26
我的siRNA阴性对照荧光和不加荧光的管子坏掉了,放在-80进水了,会不会影响使用以及siRNA效果
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0回答 2096浏览
2020-07-22
原代心肌细胞去做干扰,好做吗?干扰的基因在其他细胞系里做过的。用质粒还是病毒比较好。
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2020-07-06
 siRNA加TT作用是什么?是利于解离为单链呢还是为了稳定?
1回答 5316浏览
TT表示垂悬碱基哈

TT表示垂悬碱基哈

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    siRNA一般能表达多,最长能多久做完实验?
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