黑胶虫和支原体污染是同一个东西吗？
黑胶虫和支原体污染是同一个东西吗？
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登录后方可回帖
黑胶虫和支原体不一样。
支原体是一种大小介于细菌和病毒之间，并独立生活的原核微生物。支原体污染具有隐蔽性，它们可以与细胞长期共存，大部分细胞被污染后似乎无明显变化，培养基一般不浑浊，但其实支原体会抑制细胞生长，并且导致染色体畸变等，对细胞培养实验影响巨大。在常规光学显微镜下无法观察到，荧光显微镜下可用DAPI染色观察到支原体污染。检测支原体污染也可以使用支原体检测试剂盒进行检测。
黑胶虫是一种生物还是非生物，学术界至今还有争议，主要污染源是血清。黑胶虫污染起初对细胞并无影响，当达到一定数量时则会影响细胞生长。受黑胶虫污染的细胞液通常清亮无变化，在高倍显微镜下可以观察到点状片状的游动小体，做布朗运动。