为什么要做TA克隆（TA cloning）？为什么不能把目的基因直接连到表达载体上，然后测序，为什么一定要先连到TA克隆上然后测序在酶切连到表达载体上呢？
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登录后方可回帖
因为TA克隆简单成功率高，大多数的Taq酶都会在PCR产物的末端加一个碱基A，而T载体的缺口有一个凸出的T，正好可以配对，省去了对克隆载体和PCR产物的酶切，而且T载体连接的效率高，操作简单，可进行测序后对正确克隆扩大培养，切下目的片段，用于后续实验，这样得到的目的片段量大而且序列单一准确。